Friday, August 22, 2008

Creationism, Evolution and the Peppered Moth

Finding: The Peppered Moth has been used as an example of evolution in action. Recent controversy about the precise mechanism used to change the appearance of the Peppered Moth has allowed creationists to argue that Evolutionists have used an incorrect and fraudulent example of evolution. but new research has confirmed the actual environmental process used by evolution to change the appearance of the Peppered Moth.

An Example of Evolution in Action
For decades, the peppered moth was the textbook example of evolution in action, unassailable proof that Darwin got it right.

Recently, though, the peppered moth's status as an icon of evolution has been under threat. Emboldened by legitimate scientific debate over the fine details of the peppered moth story, creationists and other anti-evolutionists have orchestrated a decade-long campaign to discredit it - and with it the entire edifice of evolution.

These days you're less likely to hear about the peppered moth as proof of evolution than as proof that biologists cannot get their story straight.

Recent Controversy
The peppered moth now counts among the anti-evolutionists' most potent weapons. In the past few years it has helped them get material critical of evolution added to high-school science lessons in Ohio and Kansas, although the material has now been removed. In 2000, the authors of the widely used school textbook Biology reluctantly dropped the peppered moth in direct response to creationist attacks. The latest edition features the beaks of Galapagos finches instead.
Now, though, biologists are fighting back. Majerus recently finished an exhaustive experiment designed to repair the peppered moth's tattered reputation and reverse the creationists' advances. The preliminary results are out, and Majerus says they are enough to fully reinstate the moth as the prime example of Darwinian evolution in action.

The Peppered Moth Evolutionary Story
The textbook version of the peppered moth story is simple enough. Before the mid-19th century, all peppered moths in England were cream coloured with dark spots. In 1848, however, a "melanic" form was caught and pinned by a moth collector in Manchester. By the turn of the 20th century melanic moths had all but replaced the light form in Manchester and other industrial regions of England. The cause of the change was industrial pollution: as soot and other pollutants filled the air, trees used by peppered moths as daytime resting places were stripped of their lichens then stained black with soot. Light-coloured moths that were well camouflaged on lichen-coated trees were highly conspicuous on blackened trees. Melanic moths, in contrast, were less easily spotted by predatory birds and so survived longer, leaving more offspring than the light forms. As melanism is heritable, over time the proportion of black moths increased.
As with all textbook examples, however, this is a simplified account of decades of field work, genetic studies and mathematical analyses carried out by dozens of researchers. It also draws disproportionately on the flawed work of one biologist, Bernard Kettlewell of the University of Oxford.

1950 Experiments
In the 1950s Kettlewell carried out a series of classic experiments that cemented the peppered moth's iconic status. These were designed to test a hypothesis first proposed that the rise in melanism was a result of natural selection caused by differential bird predation.

Kettlewell carried out experiments in 1953 and 1955 in polluted woodland in Rubery, near Birmingham, and unspoiled woodland in rural Dorset. In the mornings he dropped hundreds of marked moths, both light and melanic, on tree trunks, where they quickly took up resting positions. In the evenings he used moth traps to recapture them. In Birmingham, he recaptured twice as many dark as light moths. In Dorset, he found the opposite, recapturing more light moths. The obvious conclusion was that light moths were more heavily predated than dark moths in Birmingham, and vice versa in Dorset.

During these experiments Kettlewell also directly observed robins and hedge sparrows eating peppered moths. As expected, the birds noticed and ate more light-coloured moths on soot-covered trees, and more melanic ones on lichen-covered trees. This was a breakthrough, as hardly anyone in Kettlewell's time believed that birds ate moths.

Kettlewell's experiments were accepted as proof that the rise of the melanic moth was a case of evolution by natural selection, and that the agent of selection was bird predation. The peppered moth quickly found its way into textbooks, often accompanied by striking photographs of light and dark moths resting on lichen-covered and soot-stained bark.

Problems with the Experiments
But in truth there were problems with Kettlewell's experiments. Perhaps the most significant was that he released moths onto tree trunks. Although moths occasionally choose trunks as a daytime resting place, they prefer the underside of branches. Kettlewell also let his moths go during the day, even though they normally choose their resting place at night. And he released more moths than would naturally be present in an area, which may have made them more conspicuous and tempted birds to eat them even if they wouldn't normally. These problems were familiar to evolutionary biologists, many of whom tried to resolve them with experiments, but were not given a general airing until 1998, when Majerus pointed out the flaws in Kettlewell's work in his book Melanism: Evolution in action.

The Origins of the Controversy
In November 1998, Nature published a review of his book by evolutionary biologist Jerry Coyne of the University of Chicago. In it, Coyne wrote a sentence that would come back to haunt him: "For the time being we must discard Biston as a well-understood example of natural selection in action." He did not mean to imply that the peppered moth was not an example of evolution by natural selection, merely that the fine details were still lacking. "I wasn't very clear. The key was well-understood."

But to anti-evolution organisations such as the Discovery Institute, they took the criticism of the Kettewell experiments. Coyne's words were taken out of context and were selectively quoting him and Majerus they managed to portray the textbook version of events as hopelessly flawed, and with it the entire theory of evolution. They also pointed at the textbook pictures - which are often staged with dead specimens - and proclaimed that the science behind those pictures was staged too.

Out of the Frying Pan into the Fire
In 2000 Majerus embarked on a large experiment designed to iron out the problems with Kettlewell's work. But things took a turn for the worse when in 2002, journalist Judith Hooper published a popular science book called Of Moths and Men: Intrigue, tragedy & the peppered moth. She accused Kettlewell of manipulating his data to prove his hypothesis. Hooper's book is not a creationist text, but creationists seized on it anyway as evidence that Kettlewell was a fraud.

Promlems with Hooper's Book
Numerous historians and scientists pointed out that Hooper's book is littered with factual errors, not least the accusation that Kettlewell forged his data. There is no evidence he did so. Coyne himself wrote a scathing review of Hooper's book in which he accused her of unfairly smearing Kettlewell and concluded that "industrial melanism still represents a splendid example of evolution in action". It is fair to say that this accurately represented the views of the vast majority of evolutionary biologists at the time, but by then the damage had been done.

Reworking the Experiments
Meanwhile, Majerus was steadily working through his experiment in his own garden in Cambridge. He started by identifying 103 branches that were suitable resting places for peppered moths, ranging in height from 2 to 26 metres, many of them covered in lichen. For seven years, every night from May to August, he placed nets around 12 randomly chosen branches and released a single moth into each net. Around 90 per cent were light-coloured to reflect the natural frequencies of the two forms around Cambridge.

The moths took up resting positions overnight, usually on the underside of the branch. At sunrise the next morning Majerus removed the nets and 4 hours later checked to see which moths were still there. His assumption was that, as peppered moths spend the whole day in their resting position, any that disappeared between sunrise and mid-morning had almost certainly been spotted and eaten by birds.

Because he was able to watch some of the branches from his house through binoculars, he also observed the moths being eaten by many species of bird - including robins, blackbirds, magpies and blue tits. As expected, the birds were better at spotting the dark moths than the camouflaged light ones, he says.

Majerus addressed all the flaws in Kettlewell's experiments. He let moths choose their own resting positions, he used low densities, he released them at night when they were normally active, and he used local moths at the frequencies found in nature.

Majerus presented his preliminary results at a meeting of evolutionary biologists at the University of Uppsala in Sweden. He said that over the seven years, 29 per cent of his melanic moths were eaten compared with 22 per cent of light ones. This was a statistically significant difference.

As in many parts of the UK, pollution in Cambridge has declined since the adoption of clean air acts in the 1950s, and melanic moths are becoming increasingly rare, declining from 12 per cent of the population in 2001 to under 2 per cent today. According to Majerus, his results show that bird predation is the agent of this change. Birds were better at spotting dark moths than light ones, ate more of them and reduced the percentage of black moths over time. It provides the proof of evolution.

There is no doubt that the peppered moth's colour is genetically determined, so changes in the frequencies of light and dark forms demonstrate changes in gene frequencies - and that is evolution. What's more, the direction and speed at which this evolution occurred can only be explained by natural selection.

Anti-evolutionists continue to suggest there is, of course, but as far as Majerus and others are concerned their claims have been debunked and the peppered moth should be reinstated as a textbook example of evolution in action. Not just to teach children either, but also as a direct rebuttal of anti-evolutionism. The peppered moth story is easy to understand because it involves things that we are familiar with: vision and predation and birds and moths and pollution and camouflage and lunch and death. That is why the anti-evolution lobby attacks the peppered moth story. They are frightened that too many people will be able to understand.

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